- Apoptosis-inducing effects of Melissa officinalis L. essential oil in glioblastoma multiforme cells.
Apoptosis-inducing effects of Melissa officinalis L. essential oil in glioblastoma multiforme cells.
Cancer investigation (2014-04-22)
Rafaela Muniz de Queiroz, Christina Maeda Takiya, Lívia Paes Tavares Pacheco Guimarães, Gleice da Graça Rocha, Daniela Sales Alviano, Arie Fitzgerald Blank, Celuta Sales Alviano, Cerli Rocha Gattass
PMID24745610
ABSTRACT
Current therapies for glioblastoma multiforme (GBM) are not effective. This study investigated the activity of the M. officinalis essential oil (EO) and its major component (citral) in GBM cell lines. Both EO and citral decreased the viability and induced apoptosis of GBM cells as demonstrated by DNA fragmentation and caspase-3 activation. Antioxidant prevented citral-induced death, indicating its dependence on the production of reactive oxygen species. Citral downmodulated the activity and inhibited the expression of multidrug resistance associated protein 1 (MRP1). These results show that EO, through its major component, citral, may be of potential interest for the treatment of GBM.
MATERIALS
Product Number
Brand
Product Description
Acetylcysteine, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Fluorescein, European Pharmacopoeia (EP) Reference Standard
Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard