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  • Improvement of Salmonella detection on motility enrichment media by ferrioxamine E-supplementation of pre-enrichment culture.

Improvement of Salmonella detection on motility enrichment media by ferrioxamine E-supplementation of pre-enrichment culture.

International journal of food microbiology (1995-10-01)
P Pless, R Reissbrodt
ABSTRACT

Supplementation of buffered peptone water with ferrioxamine E in concentrations ranging from 0.001 to 1.0 microgram/ml significantly increased the motility of Salmonella on MSRV and DIASSALM semi-solid enrichment media. Zone diameters of swarming S. enteritidis and S. typhimurium increased more than twofold following use of pre-enrichment cultures supplemented with 0.01 microgram ferrioxamine E/ml. The activity of ferrioxamine E is similar at 37 degrees C and 42 degrees C. Pre-enrichment of Salmonella in a variety of foods in supplemented buffered peptone water, with shaking at 37 degrees C for 6 h and motility enrichment at 42 degrees C for 16 h, enabled motile Salmonella to be detected in 1 day.

MATERIALS
Product Number
Brand
Product Description

Millipore
Ferrioxamine E from Streptomyces antibioticus, suitable for microbiology, ≥95%