575664-U
Supel™-Select SCX SPE 96-well Plate
bed wt. 30 mg/well, pk of 1
About This Item
Recommended Products
material
PE frit (20 μm)
polypropylene hardware
quality
MS Friendly
composition
bed wt., 30 mg/well
packaging
pk of 1
technique(s)
solid phase extraction (SPE): suitable
surface area
160-420 m2/g
matrix
Sulfonic acid functionalized hydrophilic modified styrene polymer particle platform
matrix active group
sulfonic acid phase
particle size
50-70 μm
pore size
80-200 Å pore size
application(s)
food and beverages
separation technique
ion exchange
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General description
Sample Matrix Compatibility: Aqueous solutions (biological fluids, water)
- Sulfonic acid functionalized hydrophilic modified styrene polymer
- Developed for the extraction of a highly broad range of compounds from aqueous samples
- Retention predominately based on ion-exchange; however, because the phase is hydrophilic modified, it is amenable to polar compounds as well.
Features and Benefits
- Excellent sample prep performance at a lower price
- Amenable to generic methodology - save time, money, and headache during method development
- Greater capacity allows for smaller bed weights = smaller elution volumes = time savings in sample processing
- Resistance to overdrying allows for more robust methdology
- Low UV and MS extractables for lower background and greater sensitivity
- Stringent production and QC guidelines offer greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Shown here is the chiral separation of amphetamine enantiomers under MS-compatible conditions on Astec CHIROBIOTIC® V2 after extraction from urine using Supel™-Select SCX SPE 96-well plates. The highest grade mobile phase solvents and additives were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
SPE retention mechanism in this case is based on the electrostatic attraction of charged functional groups of the analyte(s) to oppositely charged functional groups on the sorbent.
Protocols
Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine
In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine.
LC/MS Analysis of Methamphetamine Enantiomers on Astec® CHIROBIOTIC® V2 in Urine after SPE using Supel™-Select SCX
Retention occurs through polar interaction between the sorbent and analytes. Typical sample matrices that can be employed in normal-phase SPE include hydrocarbon or fatty oils diluted in a solvent like hexane, isooctane, chlorinated solvent, THF, diethyl ether, or ethyl acetate.
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