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Z0627

Sigma-Aldrich

Monoclonal Anti-ZAP-70 antibody produced in mouse

~1.5 mg/mL, clone 1E7.2, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1E7.2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

human, mouse

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1.0 μg/mL using Jurkat cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ZAP70(7535)
mouse ... Zap70(22637)

General description

Monoclonal Anti-ZAP-70 (mouse IgG1 isotype) is derived from the hybridoma 1E7.2 produced by the fusion of mouse myeloma cells and splenocytes from mice. ZAP-70 is a protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells.

Immunogen

The immunogen sequence is conjugated to KLH.
peptide corresponding to amino acids 282-307 of human ZAP-70.

Application

Monoclonal Anti-ZAP-70 antibody has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblottin
  • flow cytometry
  • immunoprecipitation
  • immunocytochemistry

Monoclonal Anti-ZAP-70 antibody is suitable for use in western blot (0.5-1.0 μg/mL using Jurkat cell extract), flow cytometry, immunocytochemistry, immunoprecipitation, microarray and indirect ELISA.

Biochem/physiol Actions

ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the zeta chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling . Lack of ZAP-70 leads to an absence of antigen-specific response and also causes impaired T cell differentiation . Anti-ZAP-70 antibody is specific for human and mouse ZAP-70 (approx. 70 kDa).
Zeta-chain-associated protein kinase70 (ZAP-70) is required for T-cell activation and is activated by(Tyrosine protein kinase) Lck-mediated phosphorylation of its tyrosine residues. Mutation of the gene that creates a defective ZAP-70 results in severe combined immunodeficiency syndrome (SCID).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation
Zhang W, et al.
Cell, 92(1), 83-92 (1998)
N Isakov et al.
The Journal of biological chemistry, 271(26), 15753-15761 (1996-06-28)
The ZAP-70 protein tyrosine kinase is essential for T cell antigen receptor (TCR)-mediated signaling. The absence of ZAP-70 results in impaired differentiation of T cells and a lack of responsiveness to antigenic stimulation. In order to study the characteristics of
Defective survival of naive CD8+ T lymphocytes in the absence of the beta3 regulatory subunit of voltage-gated calcium channels
Mithilesh K J, et al.
Nature Immunology, 10(12), 1275-1275 (2009)
A C Chan et al.
Science (New York, N.Y.), 264(5165), 1599-1601 (1994-06-10)
Protein tyrosine kinases (PTKs) play an integral role in T cell activation and differentiation. Defects in the Src-family PTKs in mice and in T cell lines have resulted in variable defects in thymic development and in T cell antigen receptor
Mithilesh K Jha et al.
Nature immunology, 10(12), 1275-1282 (2009-10-20)
The survival of T lymphocytes requires sustained, Ca(2+) influx-dependent gene expression. The molecular mechanism that governs sustained Ca(2+) influx in naive T lymphocytes is unknown. Here we report an essential role for the beta3 regulatory subunit of voltage-gated calcium (Ca(v))

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