X4251

XTT sodium salt

analytical standard, ≥90%

• Synonym(s): 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt
• Empirical Formula (Hill Notation): C₂₂H₁₆N₇NaO₁₃S₂
• CAS Number: 111072-31-2
• Molecular Weight: 673.52
• MDL number: MFCD00083517
• UNSPSC Code: 41116107
• PubChem Substance ID: 329830806
• NACRES: NA.24

Properties

• grade: analytical standard
• Quality Level: 100
• Assay: ≥90%
• technique(s): HPLC: suitable; gas chromatography (GC): suitable
• application(s): forensics and toxicology; pharmaceutical (small molecule)
• format: neat
• storage temp.: 2-8°C
• SMILES string: [Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O
• InChI: 1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1
• InChI key: JACYMBNQPPWQML-UHFFFAOYSA-M

Description

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Useful in AIDS and cancer testing.

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

Principle

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

Safety Information

• Pictograms: GHS02
• Signal Word: Danger
• Hazard Statements: H242
• Precautionary Statements: P210 - P234 - P235 - P240 - P370 + P378 - P403
• Hazard Classifications: Self-react. C
• Supplementary Hazards: EUH044
• Storage Class Code: 4.1A - Other explosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves