Home Mammalian Cell CultureNutrient Mixture F-10 Ham Formulation

Nutrient Mixture F-10 Ham Formulation

Component	N2147 [1x] g/L	N6013 g/L
Inorganic Salts
Calcium Chloride	0.0333	0.0333
Cupric Sulfate • 5H₂O	0.0000025	0.0000025
Ferrous Sulfate • 7H₂O	0.000834	0.000834
Magnesium Sulfate (anhydrous)	0.07464	0.07464
Potassium Chloride	0.285	0.285
Potassium Phosphate Monobasic (anhydrous)	0.083	0.083
Sodium Bicarbonate	—	1.2
Sodium Chloride	7.4	7.4
Sodium Phosphate Dibasic (anhydrous)	0.1537	0.1537
Zinc Sulfate • 7H₂O	0.0000288	0.0000288
Amino Acids
L-Alanine	0.009	0.009
L-Arginine • HCl	0.211	0.211
L-Asparagine • H₂O	0.01501	0.01501
L-Aspartic Acid	0.0133	0.0133
L-Cysteine • HCl • H₂O	0.035	0.035
L-Glutamic Acid	0.0147	0.0147
Glycine	0.00751	0.00751
L-Histidine • 3HCl • H₂O	0.021	0.021
L-Isoleucine	0.0026	0.0026
L-Leucine	0.0131	0.0131
L-Lysine • HCl	0.0293	0.0293
L-Methionine	0.00448	0.00448
L-Phenylalanine	0.00496	0.00496
L-Proline	0.0115	0.0115
L-Serine	0.0105	0.0105
L-Threonine	0.00357	0.00357
L-Tryptophan	0.0006	0.0006
L-Tyrosine • 2Na • 2H₂O	0.00261	0.00261
L-Valine	0.0035	0.0035
Vitamins
D-Biotin	0.000024	0.000024
Choline Chloride	0.000698	0.000698
Folic Acid	0.00132	0.00132
myo-Inositol	0.000541	0.000541
Niacinamide	0.000615	0.000615
D-Pantothenic Acid (hemicalcium)	0.000715	0.000715
Pyridoxine • HCl	0.000206	0.000206
Riboflavin	0.000376	0.000376
Thiamine • HCl	0.001	0.001
Vitamin B₁₂	0.00136	0.00136
Other
D-Glucose	1.1	1.1
HEPES	4.77	—
Hypoxanthine	0.00408	0.00408
Phenol Red (sodium)	0.0013	0.0013
Pyruvic Acid (sodium)	0.11	0.11
Thioctic Acid	0.00021	0.00021
Thymidine	0.00073	0.00073
Add
L-Glutamine	0.146	0.146

Component	N6635 g/L	N6908 [1x] g/L
Inorganic Salts
Calcium Chloride	0.0333	0.0333
Cupric Sulfate • 5H₂O	0.0000025	0.0000025
Ferrous Sulfate • 7H₂O	0.000834	0.000834
Magnesium Sulfate (anhydrous)	0.07464	0.07464
Potassium Chloride	0.285	0.285
Potassium Phosphate Monobasic (anhydrous)	0.083	0.083
Sodium Bicarbonate	—	1.2
Sodium Chloride	7.4	7.4
Sodium Phosphate Dibasic (anhydrous)	0.1537	0.1537
Zinc Sulfate • 7H₂O	0.0000288	0.0000288
Amino Acids
L-Alanine	0.009	0.009
L-Arginine • HCl	0.211	0.211
L-Asparagine • H₂O	0.01501	0.01501
L-Aspartic Acid	0.0133	0.0133
L-Cysteine • HCl • H₂O	0.035	0.035
L-Glutamic Acid	0.0147	0.0147
Glycine	0.00751	0.00751
L-Histidine • 3HCl • H₂O	0.021	0.021
L-Isoleucine	0.0026	0.0026
L-Leucine	0.0131	0.0131
L-Lysine • HCl	0.0293	0.0293
L-Methionine	0.00448	0.00448
L-Phenylalanine	0.00496	0.00496
L-Proline	0.0115	0.0115
L-Serine	0.0105	0.0105
L-Threonine	0.00357	0.00357
L-Tryptophan	0.0006	0.0006
L-Tyrosine • 2Na • 2H₂O	0.00261	0.00261
L-Valine	0.0035	0.0035
Vitamins
D-Biotin	0.000024	0.000024
Choline Chloride	0.000698	0.000698
Folic Acid	0.00132	0.00132
myo-Inositol	0.000541	0.000541
Niacinamide	0.000615	0.000615
D-Pantothenic Acid (hemicalcium)	0.000715	0.000715
Pyridoxine • HCl	0.000206	0.000206
Riboflavin	0.000376	0.000376
Thiamine • HCl	0.001	0.001
Vitamin B₁₂	0.00136	0.00136
Other
D-Glucose	1.1	1.1
HEPES	4.77	—
Hypoxanthine	0.00408	0.00408
Phenol Red (sodium)	0.0013	0.0013
Pyruvic Acid (sodium)	0.11	0.11
Thioctic Acid	0.00021	0.00021
Thymidine	0.00073	0.00073
Add
L-Glutamine	0.146	0.146

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