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G005854

LC/MS Analysis of Antiarrhythmic Drugs and Metabolites in Plasma on Ascentis® Express HILIC following Sample Prep using HybridSPE®-Phospholipid

application for HPLC

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technique(s)

HPLC: suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rabbit plasma, unfiltered K2-EDTA, spiked with each compound at 100 ng/mL (3:1, plasma:1% formic acid in acetonitrile)
SPE tube/cartridge: HybridSPE-Phospholipid, 96-well plate (575656-U)
sample addition: to each well add 100 μL plasma, followed by a 300 μL of 1% formic acid in acetonitrile
elution: collect, filtrate and analyze directly.
column: Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile, 5:95 (A:B), adjusted to pH 7.0 with formic acid
flow rate: 0.4 mL/min
pressure: 1305 psi (90 bar)
column temp.: 35 °C
detector: ESI+, 100-1000 m/z
injection: 0.5 μL

suitability

application for HPLC

application(s)

clinical

Analysis Note

This application shows the extraction and analysis of antiarrhythmic cardiac drugs in plasma samples using LC/MS/MS. HILIC chromatography on Ascentis Express along with a selective sample preparation using HybridSPE-Phospholipid provided sensitivity and resolution. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
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