H6136

Hanks′ Balanced Salts

Without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): Hanks′ Balanced Salt Solution
• MDL number: MFCD00217418
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• storage temp.: 2-8°C

Description

General description

Balanced Salt solutions (BSS) are essential in cell culture as they serve as irrigating, transporting, and diluting agents to maintain the osmotic balance between intra-and extracellular spaces. They provide cells with water, inorganic ions, and energy for normal cell metabolism. BSS also provides a buffering system to maintain the medium within the optimum pH range (7.2-7.6).

Application

Hanks′ Balanced Salt has been used:

• in the preparation of stock solution for culturing mice primary hepatocytes
• to measure the mineralized nodule formation in human osteoblast cultures
• in the preparation of embryo buffer to collect zebrafish eggs

Quantity

Formulated to contain 9.8 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves