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53B3

Application: Fusion partner, 85062501

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Bovine Heterohybridoma

ImmunoDon

Jersey calf lymph node

Myeloma

53B3 (mouse x bovine heteromyeloma)

Reactivity

Application: Fusion partner

technique(s)

cell culture | mammalian: suitable

isotype

Not specified

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse x Bovine 53B3 x Jersey calf lymph node

Cell Line Description

This HAT sensitive line can be used as a fusion partner in the production of heterohybridomas.

Immunogen

Sheep red blood cells

Application

Fusion partner

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Hybridoma Fusion Partners Cell Lines

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines at sigmaaldrich.com

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