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07-681

Sigma-Aldrich

Anti-phospho-AMPK α (Thr172) Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_006251.5
NM_206907.3

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species reactivity

guinea pig, rabbit, rat, human, mouse, canine

species reactivity

rabbit, human, mouse

species reactivity

mouse, human

species reactivity

rat, human, mouse, fish

antibody form

culture supernatant

antibody form

purified antibody

antibody form

purified antibody

antibody form

ascites fluid

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

wet ice

shipped in

dry ice

shipped in

ambient

shipped in

dry ice

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

-

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable

technique(s)

indirect immunofluorescence: 1:100 using freshly dissected and frozen human or animal muscle tissue., microarray: suitable

Specificity

Recognizes phospho-AMPKα.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Thr172 of human AMP-activated protein kinase alpha 2 (AMPKα2)

Application

Anti-phospho-AMPK α (Thr172) Antibody is an antibody against phospho-AMPK α (Thr172) for use in WB.

Quality

routinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK

Target description

Mr 63 kDa

Physical form

Format: Purified

Analysis Note

Control
Serum starved HEK293 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

    • Certificates of Analysis (COA)

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    J F P Wojtaszewski et al.
    Biochemical Society transactions, 31(Pt 6), 1290-1294 (2003-12-04)
    The AMPK (5'AMP-activated protein kinase) is becoming recognized as a critical regulator of energy metabolism. However, many of these effects in muscle metabolism have been ascribed to AMPK based on the use of the unspecific activator AICAR (5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside). Using mouse
    Roles of 5'-AMP-activated protein kinase (AMPK) in mammalian glucose homoeostasis.
    Rutter, Guy A, et al.
    The Biochemical Journal, 375, 1-16 (2003)
    Mathias Fasshauer et al.
    Biochimie, 86(11), 779-784 (2004-12-14)
    Several adipocyte-secreted factors have been demonstrated to potentially link obesity, insulin resistance, and cardiovascular disease. Among those, adiponectin is an insulin-sensitizing and anti-inflammatory adipokine, concentrations of which are decreased in obesity-associated metabolic and vascular disorders. Recently, two adiponectin receptors (AdipoR)
    Tue L Nielsen et al.
    Molecular genetics and metabolism, 123(1), 21-27 (2017-11-28)
    McArdle disease (glycogen storage disease type V) is an inborn error of skeletal muscle metabolism, which affects glycogen phosphorylase (myophosphorylase) activity leading to an inability to break down glycogen. Patients with McArdle disease are exercise intolerant, as muscle glycogen-derived glucose
    Enhanced external counterpulsation improves peripheral artery function and glucose tolerance in subjects with abnormal glucose tolerance.
    Martin, JS; Beck, DT; Aranda, JM; Braith, RW
    Journal of Applied Physiology (1985)
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