Qualitative Thin Layer Chromatography Analysis of Flavonoids and Quantification of Terpene Lactones in Ginkgo Biloba Extracts and Tablets

Ginkgo is one of the oldest medicinal plants on earth. Originally it comes from China but nowadays it is cultivated all over the world. The plant contains various flavonoids and terpenes, which are responsible for the positive properties of Ginkgo. The ingredients protect the cells in the human body and promote blood circulation. Ginkgo preparations are therefore used against various diseases, such as dementia, headaches, circulatory disorders and asthma.

Results

Screening

3.0 µL of different standards and samples were applied on the plate as bands. After staining with the natural substance reagent according to Neu (1% 2-aminoethoxydiphenyl borate in methanol) and heating (5 minutes at 120 °C on a heat plate), a variety of flavonoids and additional substances became visible. Most of them are shown on the picture at a wavelength of 366 nm. At 254 nm the first tablet (track 6) shows a spot at the same position as the theobromine standard (track 2). A mass spectrometry (MS) analysis only reveals the existence of theobromine in the standard (track 2) theobromine. No theobromine is present in the first tablet (track 6). The first tablet displays the highest concentration of ingredients as compared to the Ginkgo powder amount and application volume of the other tablets. Tablets 1, 2 and 3 contain 500, 500 and 6.000 mg of dried Ginkgo leaf extract, respectively. The Ginkgo Biloba peak identification standard (track 10) shows a good separation of several flavonoids. Taken it as a fingerprint standard, the tablets and the tea contain nearly the same substances.

Quantification

Before the plate was used for the separation, it was impregnated with a 5% sodium acetate solution (dissolved in ethanol / water 6:4) and dried at 120 °C. The standard was applied in a concentration series as bands together with the samples on one plate. After the chromatographic development, the plate was derivatized with acetic anhydride and heated at 180 °C for 10 minutes in order to make the terpenes visible under UV light. Then the spots were scanned at 320 nm and the peaks were integrated.

The results show that the tablets, which all appear similar in their look, contain different levels of flavonoids and terpenes. This means that manufacturers yield their Ginkgo leaves from different growing regions. Tablet 3 contains the highest level of terpenes as compared to the other two samples.

MS Analysis

A HPTLC silica gel 60 F₂₅₄ MS-grade plate was used for the TLC-MS experiments. For the analysis of terpene lactones the samples were eluted with the Camag TLC-MS Interface from the developed and underivatized plate and transferred to an ESI(-) Q-TOF-MS from Bruker.

The spectra clearly show mass peaks for bilobalide, ginkgolide A and ginkgolide B.

Sample preparation

Tea and tablets were diluted in methanol / water (1:1) and sonicated. Subsequently the samples were filtered through a syringe filter and the filtrate was collected in small tubes.

Figure 1. Syringe Filter

Screening of Flavonoids

Table 1

Plate	HPTLC silica gel 60 F254
Mobile Phase	ethyl acetate / water / methanol / n-heptane 16:1:3:2
Sample Solvent	methanol / water 1:1
Solutions Applicated as Bands	bandwidth 6.0 mm		x = 15.0 mm	y = 10.0 mm
Migration Distance	5.0 cm
Derivatization Reagent	2-aminoethyl diphenylborinate 5% in ethyl acetate
Migration Time	18 min 30 sec
Application	Track 1	3.0 µL		Chrysin standard 1.0 mg/mL
	Track 2			Theobromine standard 1.0 mg/mL
	Track 3			Naringenin standard 1.0 mg/mL
	Track 4			Quercetin standard 1.0 mg/mL
	Track 5			Kaempferol standard 1.0 mg/mL
	Track 6			Tablet 1 100 mg/mL
	Track 7			Tablet 2 400 mg/mL
	Track 8			Tablet 3 50 mg/mL
	Track 9			Ginkgo Tea 100 mg/mL
	Track 10			Ginkgo peak identification standard 10 mg/mL

Figure 1. Plate at 254 nm

Figure 2. Derivatized plate at 366 nm

Quantification of Terpene Lactones

Table 2

Plate	HPTLC silica gel 60 F254
Pre-chromatographic derivatization	sodium acetate 5 % in ethanol / water (6:4)
Mobile Phase	toluene / ethyl acetate / acetone / methanol 100:50:50:6
Dilution solvent	methanol / water 1:1
Solutions applicated as bands	bandwidth 6.0 mm	x = 15.0 mm	y = 8.0 mm
Migration distance	5.0 cm
Migration time	11 min
Derivatization	acetic anhydride, heating at 180°C (10 minutes)
Scanning wavelength	320 nm
Slit dimension	4.00 x 0.30 mm
MS extraction solvent	acetonitrile / water 95:5 + 1.0 % Formic acid
TLC-MS Interface (Camag)	Flow rate 0.2 ml/min
MS (Bruker)	ESI (-) Q-TOF-MS

Table 3

Compound	hRf	Conc. [mg/mL]	App. volume [µL]	Calculated amount of terpene [µg]	SDV [%]
Terpene standard mixture	47, 36, 24	0.10	1.0	-	-
			3.0
			5.0
Bilobalide
Ginkgo tea extract	47	100.0	5.0	86.85	1.79
Ginkgo peak identification standard	47	10.0	1.5	359.06	1.37
Tablet 1	47	50.0	5.0	40.05	4.66
Tablet 2	47	400.0	3.5	127.20	4.56
Tablet 3	47	100.0	2.5	124.51	1.28
Ginkgolide A
Ginkgo tea extract	36	100.0	5.0	25.33	2.63
Ginkgo peak identification standard	36	10.0	1.5	150.83	3.71
Tablet 1	36	50.0	5.0	43.18	2.50
Tablet 2	36	400.0	>3.5	50.14	2.43
Tablet 3	36	100.0	2.5	53.58	4.37
Ginkgolide B
Ginkgo tea extract	24	100.0	5.0	-	-
Ginkgo peak identification standard	24	10.0	1.5	-	-
Tablet 1	24	>50.0	5.0	28.93	3.27
Tablet 2	24	400.0	3.5	-	-
Tablet 3	24	100.0	2.5	53.58	2.36

Figure 3. Picture at 366 nm

Figure 4. Screening

Figure 5. Mass spectra: Bilobalide 326.3 [M-H]- = 325.0730, [M-H2O-H]- = 306.8899

Figure 6. Ginkgolide A 408.4, [M-H]- = 407.1363

Figure 7. Ginkgolide B 424.4, [M-H]- = 423.1342