LC/MS Analysis of Sertraline and Norsertraline on Ascentis^® Express RP-Amide

column

Ascentis Express RP Amide, 10 cm x 3.0 mm I.D., 2.7 μm particles (53918-U)

mobile phase

[A] 10 mM ammonium formate, water, pH 3.6; [B] 10 mM ammonium formate, acetonitrile, pH 3.6; (65:35, A:B)

flow rate

0.6 mL/min

column temp.

55 °C

detector

ESI(+), 100-1000 m/z

injection

2 μL

sample

200 ng/mL in 25:75, water: acetonitrile