HPLC Analysis of Phenolic Compounds on Ascentis^® Express 2.7 μm RP-Amide

column

Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)

mobile phase

[A] 20 mM phosphoric acid, pH 2; [B] acetonitrile; (75:25, A:B)

flow rate

1.5 mL/min

column temp.

35 °C

detector

UV, 270 nm

injection

25 μL

sample

50 mg/L in mobile phase