HPLC Analysis of Glutethimide Enantiomers on Astec^® Cellulose DMP

column

Astec Cellulose DMP, 25 cm x 4.6 mm I.D., 5 μm particles (51099AST)

mobile phase

[A] heptane; [B] isopropanol; (80:20, A:B)

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

15 μL

sample

glutethimide, 1 g/L in heptane:isopropanol (50:50)