Binding properties of HABA-type azo derivatives to avidin and avidin-related protein 4.

Binding properties of HABA-type azo derivatives to avidin and avidin-related protein 4.

Chemistry & biology (2006-10-21)

Susanna Repo, Tiina A Paldanius, Vesa P Hytönen, Thomas K M Nyholm, Katrin K Halling, Juhani Huuskonen, Olli T Pentikäinen, Kari Rissanen, J Peter Slotte, Tomi T Airenne, Tiina A Salminen, Markku S Kulomaa, Mark S Johnson

PMID17052607

ABSTRACT

The chicken genome encodes several biotin-binding proteins, including avidin and avidin-related protein 4 (AVR4). In addition to D-biotin, avidin binds an azo dye compound, 4-hydroxyazobenzene-2-carboxylic acid (HABA), but the HABA-binding properties of AVR4 are not yet known. Differential scanning calorimetry, UV/visible spectroscopy, and molecular modeling were used to analyze the binding of 15 azo molecules to avidin and AVR4. Significant differences are seen in azo compound preferences for the two proteins, emphasizing the importance of the loop between strands beta3 and beta4 for azo ligand recognition; information on these loops is provided by the high-resolution (1.5 A) X-ray structure for avidin reported here. These results may be valuable in designing improved tools for avidin-based life science and nanobiotechnology applications.

MATERIALS

Product Number

Brand

Product Description

54793

Supelco

2-(4-Hydroxyphenylazo)benzoic acid, matrix substance for MALDI-MS, ≥99.5%

H5126

Sigma-Aldrich

2-(4-Hydroxyphenylazo)benzoic acid, ≥90% (HPLC)