- Multifocal diffusion of a KPC-3 producing ST512 K. pneumoniae clone in Northern Italy.
Multifocal diffusion of a KPC-3 producing ST512 K. pneumoniae clone in Northern Italy.
The new microbiologica (2013-02-26)
Roberta Migliavacca, Elisabetta Nucleo, Sara Asticcioli, Erminia Casari, Silvia Bracco, Maria C Sironi
PMID23435825
ABSTRACT
Sequence Type 258 (ST258) together with its allelic single- and double-locus variants have mostly been associated with the dissemination of KPC-producing Klebsiella pneumoniae in Europe. A total of 56 nonreplicate K. pneumoniae isolates with decreased carbapenem-susceptibility, collected at 7 different hospitals located in Northern Italy were investigated for the occurrence of blaKPC-type genes. PCR and sequencing results highlighted the presence of blaKPC2 or blaKPC-3 determinants in 10/56 and 5/56 cases respectively. Here we describe the intra- and inter-hospital spread in Northern Italy of a K. pneumoniae ST512 clone harboring the blaKPC-3 gene.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Penicillinase from Bacillus cereus, lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)
Sigma-Aldrich
β-Lactamase from Enterobacter cloacae, Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)
Sigma-Aldrich
β-Lactamase from Enterobacter cloacae, Type III, lyophilized powder, 6-18 units/mg protein (using benzylpenicillin)
Sigma-Aldrich
β-Lactamase from Pseudomonas fluorescens, lyophilized powder, recombinant, expressed in E. coli