Residue investigation of some phenylureas and tebuthiuron herbicides in vegetables by ultra-performance liquid chromatography coupled with integrated selective accelerated solvent extraction-clean up in situ.

Some trace amounts of urea herbicide residues can be transferred to humans via the food chain, thereby being potentially harmful to human health. The development of a robust analytical methodology for effective sample preparation and simultaneous determination of herbicide residues in vegetable samples is required for achieving food safety. The diuron-molecularly imprinted polymers (MIPs) synthesized have excellent affinity and high selectivity to phenylureas (monolinuron, isoproturon, diuron and linuron) and tebuthiuron. A novel automated procedure with better selectivity for vegetable sample treatment was developed by integrated matrix solid-phase dispersion-accelerated solvent extraction clean-up in situ. Five herbicides can be baseline separated with runtime down to 5 min by ultra-performance liquid chromatography, and good linearity was obtained with a correlation coefficient (r) of 0.9999. The limit of quantification of the method was in the range of 0.8-2.3 µg kg-1 . Diuron residue in cherry tomato sample was found to be 40 µg kg-1 . The developed method has satisfactory selectivity, good linearity, high sensitivity and accuracy as well as speediness, and can ensure rapid selective extraction and sensitive multi-residue analysis at low microgram per kilogram levels of the herbicides in vegetable food. © 2018 Society of Chemical Industry.