Skip to Content
Merck
HomeProductsMolecular Biology & Functional GenomicsNucleic Acid PurificationgDNA Purification

gDNA Purification

GenElute™-E Single Spin DNA purification kit for genomic DNA

DNA purification kits are used to extract and isolate DNA from samples, removing biological and chemical impurities such as cell debris, serum, proteins, lipids, and ionic components. DNA purification kits are also used to clean and prepare DNA samples for use in various downstream applications including cloning, gene editing, in situ hybridization, PCR, qPCR, multiplex PCR, sequencing, and next generation sequencing (NGS).

Read more about

GenElute™ DNA Purification Kits

Classic GenElute™ DNA purification kits use a silica resin to strongly bind DNA under high salt conditions, allowing proteins, small RNAs, and other molecules to be removed through wash steps using a salt/ethanol solution. After washing, purified DNA is eluted in water or TE buffer. A broad portfolio of GenElute™ DNA purification kits tailored to specific sample types are available, enabling purification of genomic DNA (gDNA) from cell culture, mammalian tissue, blood, bacteria, viruses, plant tissue, soil, water, urine, and stool. GenElute™ Cell-Free DNA Kits provide rapid and efficient purification of circulating free DNA (cfDNA) to recover cell-free DNA fragments in the range of 100 bp – 500 bp, suitable for a wide range of downstream applications including next-generation sequencing, qPCR and bisulfite sequencing.

Features:

  • Silica-based DNA purification method
  • High yield, high purity DNA at lowest cost per prep
  • Most common technique used for DNA purification in laboratory workflows
  • ~90-minute protocol (varies based on lysis time)
  • Comprehensive portfolio of kits tailored for specific sample types
  • Vacuum and spin formats
  • Multi-analyte (dual RNA/DNA copurification kits) available

GenElute™-E Single Spin DNA Purification Kits

GenElute™-E DNA purification kits use a negative chromatography technique dependent on size exclusion to isolate and purify nucleic acid in a single spin. In size exclusion chromatography, molecules are separated by size. Smaller molecules such as proteins, lipids, and ionic components become trapped in the pores of the stationary phase of the column. Larger DNA molecules flow through the column quickly because they are too large to enter the pores. By exploiting this difference in size, GenElute™-E purification kits enable DNA purification in a single spin without the need for multiple binding and wash steps. 

Features:

  • Negative chromatography DNA purification method based on size exclusion
  • Simplified workflow
  • Quick protocol (3 minutes hands-on time, for a total of 15-45 minutes depending on lysis time)
  • Fewer chemical impurities, with no chaotropic salts or alcohol wash solutions that can inhibit downstream processes such as PCR
  • Fewer biological impurities, with less shearing of genomic DNA due to reduced number of spin steps
  • Reduced plastic waste and no hazardous binding or wash buffers
  • Eco-friendly packaging
  • Easy-to-follow protocols and checklists
  • Portfolio of kits tailored for genomic DNA purification, PCR cleanup, and gel extraction

EXTRACT-N-AMP™ PCR KITS

Extract-N-Amp™ PCR kits are the first integrated DNA extraction and amplification kits for blood, tissue, and plant samples. These innovative kits provide all the reagents necessary to rapidly release DNA and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. Kits also include a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract. 

Features:

  • Integrated purification and amplification 
  • Simplified sample processing
  • Kits include all reagents, proprietary buffers, and enzymes needed to rapidly extract and amplify targets of interest from a variety of cells and tissues
  • REDExtract-N-Amp™ PCR ReadyMix™ contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reaction onto agarose gels for analysis

Related Product Resources

Page 1 of 2

Related Videos

Slide 1 of 4
GenElute™-E Demo Video
GenElute™-E Demo Video
GenElute-E DNA and RNA Purification Kits
Webinar: Is Your PCR Reaction Healthy?
Webinar: Is Your PCR Reaction Healthy?
In this webinar, Katherine Mechling discusses what to do when things go wrong with PCR.
DNA Purification with GenElute™-E Single Spin Technology
DNA Purification with GenElute™-E Single Spin Technology
Silicon-based DNA purification can be tedious. Let's try something different.
REDExtract-N-Amp™ Tissue PCR Kit
REDExtract-N-Amp™ Tissue PCR Kit
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
Related Product Categories
Plasmid DNA Purification
Simplify plasmid DNA purification with kits in multiple sizes. Ideal for cloning and protein expression needs.
RNA Purification
Rapid, reliable total RNA and mRNA purification from a variety of samples including cells, tissues, viruses, plants, serum, and plasma.
Nucleic Acid Gel Electrophoresis Equipment and Systems
Optimize DNA and RNA analysis with our stains, gels, and labeling reagents for precise quantitation.
Molecular Cloning & Protein Expression
Molecular cloning and protein expression methods and protocols for reliable cloning and robust protein expression from insect, bacteria, and mammalian protein expression vectors.
Transfection
Transfection reagents, protocols, and resources including Roche X-tremeGENE transfection reagents for DNA, siRNA, miRNA, and CRISPR/Cas9 gene editing components.
PCR Reagents & Kits
Master PCR for Discovery: Amplify DNA exponentially with our reagents, supporting gene analysis, sequencing, mutagenesis, and more.
Custom & Predesigned DNA Oligos and qPCR Probes
Discover our custom DNA oligos portfolio including primers, qPCR probes, high-throughput DNA in plates, and next-generation sequencing (NGS) adapters available in multiple formats that comply with ISO 9001 and ISO 13485 quality systems for research and commercial applications.
Whole Genome Amplification
Whole Genome Amplification (WGA) kits, reagents, and protocols to support your WGA workflow.
Related Applications
DNA & RNA Purification
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Cloning & Expression
Cloning, protein expression vectors and techniques for optimal gene cloning and protein expression systems.
PCR Applications
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
qPCR
Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.
Next Gen Sequencing
Next-Generation Sequencing (NGS) reagents and resources for massively parallel sequencing applications.
Sequencing
DNA sequencing reagents and tools for Sanger sequencing methods, DNA primers, probes, and additional nucleic acid sequencing applications.
Nucleic Acid Labeling & Detection
DNA and RNA nucleic acid labeling and detection methods and applications for northern blot, Southern blot, and additional nucleic acid detection assays.
Nucleic Acid Gel Electrophoresis
Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge for cloning, PCR, mass spectrometry, next generation sequencing (NGS), and Northern and Southern blotting applications.
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?