82021-U
Hamilton PRP-1 HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.1 mm, stainless steel hardware
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About This Item
UNSPSC Code:
41115700
NACRES:
SB.52
Recommended Products
material
stainless steel column
Agency
suitable for USP L21
manufacturer/tradename
Hamilton
packaging
1 ea of
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.1 mm
matrix
PS-DVB particle platform
polymeric
matrix active group
polymer
particle size
5 μm
pore size
100 Å
operating pH
1-14
separation technique
reversed phase
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General description
PRP-1 is a co-polymer of poly (styrene-divinylbenzene). Its surface is chemically neither homogenous nor stable during use. Hamilton PRP-1 HPLC Column, is used for large scale purification of oligonucleotides, generally used in NMR studies.
The Hamilton PRP-1, polymeric reversed phase columns are Ideal for high pH (pH 8-13) operation or with analytes that give poor chromatogrpahy on silica-based columns.
Application
Hamilton PRP-1 HPLC Column was used in experimental studies employing liquid chromatography (LC); with reversed phase separation (RPLC) using only pressurized hot water (PHW) as a mobile phase. It also is suitable for being used in large-scale purification of synthetic oligonucleotides, with a phosphate/methanol/acetonitrile solvent system.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Find documentation for the products that you have recently purchased in the Document Library.
Characterization of some commercial poly (styrene-divinylbenzene) copolymers for reversed-phase HPLC1.
Stuurman HW
Chromatographia, 23 (5), 341-349 (1987)
Large-scale purification of synthetic oligonucleotides and carcinogen-modified oligodeoxynucleotides on a reverse-phase polystyrene (PRP-1) column.
Guanjin H and Krugh TR.
Analytical Biochemistry, 190 (1), 21-25 (1990)
Liquid chromatography at elevated temperatures with pure water as the mobile phase.
Kari H and Riekkola ML.
Trends in Analytical Chemistry, 27 (1), 1-14 (2008)
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