567212-U

Discovery^® BIO Wide Pore C18 HPLC Column

10 μm particle size, L × I.D. 25 cm × 21.2 mm

• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L1
• product line: Discovery^®
• feature: endcapped
• manufacturer/tradename: Discovery^®
• packaging: 1 ea of
• extent of labeling: 9.2% Carbon loading
• parameter: 0-70 °C temperature; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 21.2 mm
• surface area: 100 m²/g
• surface coverage: 3.6 μmol/m²
• matrix: silica particle platform; fully porous particle
• matrix active group: C18 (octadecyl) phase
• particle size: 10 μm
• pore size: 300 Å
• operating pH: 2-8
• separation technique: reversed phase

Description

General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany