GE17-5079-01
HiTrap® rprotein A Fast Flow
Cytiva 17-5079-01, pack of 5 × 1 mL
About This Item
Recommended Products
ligand
recombinant protein A (E. coli)
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5079-01
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
4% cross-linked agarose
particle size
60-165 μm
average diameter
90 μm (d50v)
cleaning
2-11(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-10
capacity
~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
Related Categories
General description
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
Features and Benefits
- Quick, convenient preparative purification of monoclonal antibodies.
- Well-established protein A-based medium used in many approved MAb processes.
- Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
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