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189724

Sigma-Aldrich

Ascorbate Oxidase, Cucurbita sp.

Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.

Synonym(s):

Ascorbate Oxidase, Cucurbita sp.

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352202

Quality Level

form

lyophilized

specific activity

≥1500 U/mg
≥300 units/mg material

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

shipped in

ambient

storage temp.

−20°C

General description

Native ascorbate oxidase from Cucurbita species. A multi-copper-containing oxidase that catalyzes oxidation of ascorbic acid to dehydroascorbic acid. AO catalyses the four-electron reduction of dioxygen to water with concominant one-electron oxidation of the reducing organic substrate. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid. Inhibited by azide, dithiocarbamate, and thiocyanate.
Native ascorbate oxidase from Cucurbita species. Catalyzes oxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid. Inhibited by azide, dithiocarbamate, and thiocyanate.

Caution

enzyme will denature below pH 4.0.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol ascorbic acid per min at 37°C, pH 5.6.

Reconstitution

Following reconstitution refrigerate (4°C). This Stock solutions are stable for up to 3 weeks at 4°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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