1.00213
GranuCult® prime Sorbitol MacConkey (SMAC) Agar
acc. FDA-BAM, ISO 16654
Synonym(s):
CT-SMAC Agar, MacConkey Agar with Sorbitol, SMAC Agar
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About This Item
Recommended Products
Agency
ISO 16654
Quality Level
form
powder
technique(s)
microbiological culture: suitable
pH
7.1±0.2 (25 °C)
application(s)
food and beverages
pharmaceutical
storage temp.
15-25°C
suitability
selective and differential for Escherichia 0157:H7
General description
GranuCult® prime Sorbitol MacConkey (SMAC) Agar is a selective agar medium to isolate and differentiate enterohemorrhagic (EHEC) E. coli 0157 from food, animal feed and other samples.
The presence of bile salts and crystal violet inhibits the growth of gram-positive microbes thereby enhancing the growth of gram-negative microorganisms.
The specific selectivity of the medium is done by the addition of Cefixime potassium Tellurite supplement towards E. coli 0157:H7 and in turn suppresses the accompanying flora. Addition of sorbitol in the medium along with pH indicator neutral red is used to detect the sorbitol positive colonies which appear red in color. Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.
Enzymatic digest of casein and animal tissue provides nitrogen, vitamins, and amino acids to the growing microbes. Agar is the solidifying agent.
The presence of bile salts and crystal violet inhibits the growth of gram-positive microbes thereby enhancing the growth of gram-negative microorganisms.
The specific selectivity of the medium is done by the addition of Cefixime potassium Tellurite supplement towards E. coli 0157:H7 and in turn suppresses the accompanying flora. Addition of sorbitol in the medium along with pH indicator neutral red is used to detect the sorbitol positive colonies which appear red in color. Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.
Enzymatic digest of casein and animal tissue provides nitrogen, vitamins, and amino acids to the growing microbes. Agar is the solidifying agent.
Application
GranuCult® prime Sorbitol MacConkey (SMAC) Agar is used for the isolation and differentiation of sorbitol negative Escherichia coli serotype O157 from food, animal feed and other test samples.
Analysis Note
- The dehydrated media is granulated with light pink color.
- Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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