LC/MS Analysis of Polar Amino Acids on Ascentis® Si (HILIC Mode)
![LC/MS Analysis of Polar Amino Acids on Ascentis® Si (HILIC Mode) application for HPLC](/deepweb/assets/sigmaaldrich/product/images/124/424/2975728d-6f7e-4300-bddf-6143b1d6c698/800/2975728d-6f7e-4300-bddf-6143b1d6c698.jpg)
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
L-Asparagine
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandL-Threonine
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandCONDITIONS
column
Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)
mobile phase
[A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] water; [C] acetonitrile
gradient
5% A constant; 10 to 16% B, 85 to 79% C in 20 min
flow rate
0.3 mL/min
column temp.
35 °C
detector
ESI(+)
injection
2 μL
sample
0.1 g/L each in mobile phase
Description
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany