HPLC Analysis of mAb F on Zenix®-300 and Zenix®-C 300
CONDITIONS
column
Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)
column
Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)
mobile phase
150 mM sodium phosphate, pH 7.0
flow rate
1.0 mL/min
pressure
1073 psi (74 bar)
column temp.
25 °C
detector
UV, 280 nm
injection
10 μL
sample
1.23 mg/mL mAb F in 10 mM sodium succinate, pH 5.0
Description
Legal Information
Zenix is a registered trademark of Sepax Technologies