- Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.
Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.
Bioorganic & medicinal chemistry letters (2000-12-29)
J E Sheppeck, H Kar, L Gosink, J B Wheatley, E Gjerstad, S M Loftus, A R Zubiria, J W Janc
PMID11128641
ABSTRACT
A statistically exhaustive, 8800 compound tripeptidal amidomethylcoumarin library was synthesized as discreet compounds using solid-phase combinatorial chemistry. A subset of the compounds was purified by HPLC and tested in a high-throughput fluorometric assay against several known serine and cysteine proteases to demonstrate the utility of this library for profiling protease substrate specificity.