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Nanobioconjugates of Candida antarctica lipase B and single-walled carbon nanotubes in biodiesel production.

Nanobioconjugates of Candida antarctica lipase B and single-walled carbon nanotubes in biodiesel production.

Bioresource technology (2015-11-23)

László Csaba Bencze, Judith H Bartha-Vári, Gabriel Katona, Monica Ioana Toşa, Csaba Paizs, Florin-Dan Irimie

ABSTRACT

Carboxylated single-walled carbon nanotubes (SWCNTCOOH) were used as support for covalent immobilization of Candida antarctica lipase B (CaL-B) using linkers with different lengths. The obtained nanostructured biocatalysts with low diffusional limitation were tested in batch mode in the ethanolysis of the sunflower oil. SWCNTCOOH-CaL-B proved to be a highly efficient and stable biocatalyst in acetonitrile (83.4% conversion after 4h at 35°C, retaining >90% of original activity after 10 cycles).

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, Absolute - Acetone free

Sigma-Aldrich

Methanol, Laboratory Reagent, ≥99.6%

Sigma-Aldrich

Heptadecanoic acid, ≥98%

Sigma-Aldrich

Eicosane, 99%

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

Sigma-Aldrich

Methanol, NMR reference standard

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, BioReagent, ≥99.93%

Sigma-Aldrich

Methyl oleate, technical grade, 70%

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Hexamethylenediamine, 98%

Sigma-Aldrich

Methyl oleate, 99%

Sigma-Aldrich

1,3-Diaminopropane, ≥99%

Sigma-Aldrich

Hexamethylenediamine, technical grade, 70%

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Methanol-¹²C, 99.95 atom % ¹²C

Sigma-Aldrich

Methanol solution, (Methanol:Dimethyl sulfoxide 1:1 (v/v))

Sigma-Aldrich

Methanol solution, NMR reference standard, 4% in methanol-d₄ (99.8 atom % D), NMR tube size 3 mm × 8 in.

Sigma-Aldrich

Methyl arachidate, ≥99% (capillary GC)

Sigma-Aldrich

Chloroform-d, 99.8 atom % D, contains 0.03 % (v/v) TMS

Sigma-Aldrich

Chloroform-d, "100%", 99.96 atom % D, contains 0.03 % (v/v) TMS

Sigma-Aldrich

Chloroform-d, ≥99.8 atom % D, contains 0.5 wt. % silver foil as stabilizer