SAB4200787
Anti-Secretory Component (IgA) antibody, Mouse monoclonal
clone GA-1, purified from hybridoma cell culture
Synonym(s):
Anti-Secretory IgA
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
mouse
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
GA-1, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1 mg/mL
technique(s)
indirect ELISA: 0.06-0.12 μg/mL using 5 μg/ml secretory human IgA for coating.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
The secretory component is a single chain glycoprotein which is synthesized principally by epithelial cells in mucous membranes and exocrine glands. It occurs both in a free form and as a subunit of the secretory immunoglobulin A (SIgA) molecule. It has a molecular weight of approximately 75,000 Daltons. The secretory component is attached to the immunoglobulin molecule during the secretion process. Its biological function has not been established although several possibilities for which there are varying degrees of support have been suggested. These possibilities include: protection of IgA against destruction by proteolysis, transport of IgA across the epithelial surface and attraction of circulating lymphocytes with surface IgA to mucous membranes. Secretory IgA is also present in circulating blood and high concentrations of SIgA or free secretory component are reported in patients with carcinomas and chronic infectious diseases. Human IgA accounts for approximately 20% of all immunoglobulins found in adult human serum. It consists of two heavy chains and two light chains. In serum it is usually found as monomeric but in secretions it exists as a dimer linked by a J-chain and associated by a peptide secretory component. Although IgA has been shown to have the usual antibody properties it is probably more important in secretions (saliva, colostrum, tears, nasal, bronchial and intestinal) where it has the role of creating an immune barrier against various microorganisms at exposedmucous surfaces. Monoclonal Anti-Secretory Component (IgA) may be used for quantitative determination of human secretory component or secretory IgA in various body fluids and immunohistochemical localization of secretory component in mucous membrane tissue.
Immunogen
Secretory component purified from human colostrum.
Application
Indirect ELISA: a working concentration of 0.06-0.12 μg/mL is recommended using 5 μg/mL secretory human IgA for coating.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.
Other Notes
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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J Mestecky et al.
Journal of immunological methods, 193(2), 103-148 (1996-06-21)
51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition
J Weicker et al.
Journal of immunology (Baltimore, Md. : 1950), 114(4), 1337-1344 (1975-04-01)
Human secretory component (SC) was isolated from colostral whey, and the binding of 125I-SC to purified IgA and IgM monoclonal proteins was studied using two methods to separate free from immunoglobulin-bound 125I-SC: a) gel filtration on Sephadex G-200, and b)
Y Ishiguro et al.
Clinica chimica acta; international journal of clinical chemistry, 116(2), 237-243 (1981-10-26)
Highly sensitive solid-phase immunoassay systems for human immunoglobulin A (IgA), secretory component (SC), secretory immunoglobulin A (SIgA) were developed by use of antisera against the alpha-chain of IgA and SC, and beta-D-galactosidase from Escherichia coli as label. IgA and SC
R Jefferis et al.
Immunology letters, 10(3-4), 223-252 (1985-01-01)
Seventy-four monoclonal antibodies (McAb) of putative specificity for human IgG (11), the IgG sub-classes (59) or Gm allotypes (4) have been evaluated for reactivity and specificity in eight laboratories employing different assay techniques or protocols. For the IgG, IgG3, IgG4
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