G8673
β-Glucanase 2, thermostable
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym(s):
β-Glucanase, thermostable
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About This Item
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recombinant
expressed in E. coli
Assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥1.0 units/mg protein
mol wt
38 kDa
concentration
≥20 mg protein/mL (Bradford)
shipped in
wet ice
storage temp.
2-8°C
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Application
β-Glucanase is used to study cell wall modifications and for carbohydrate hydrolysis. It has been used to supplement barley-based diets for poultry and to study the reopening signal conduits and release of dormancy in the Populus species.
Biochem/physiol Actions
β-Glucanase is involved in cell wall modifications and carbohydrate hydrolysis.
Unit Definition
One unit will produce 1 μmole of reducing sugar (measured as glucose) from Beta-glucan per minute at pH 5.8 at 70 °C.
Physical form
Supplied as a solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Encarnación Dueñas-Santero et al.
Eukaryotic cell, 9(11), 1650-1660 (2010-09-21)
In yeast, enzymes with β-glucanase activity are thought to be necessary in morphogenetic events that require controlled hydrolysis of the cell wall. Comparison of the sequence of the Saccharomyces cerevisiae exo-β(1,3)-glucanase Exg1 with the Schizosaccharomyces pombe genome allowed the identification
T Ribeiro et al.
Poultry science, 90(6), 1245-1256 (2011-05-21)
To improve the nutritive value of barley-based diet for broilers, 2 experiments using 2 different barley lots were performed to evaluate the capacity of a mesophilic cellulase when fused to a β-glucan specific family 11 carbohydrate-binding module. The data revealed
Päivi L H Rinne et al.
The Plant cell, 23(1), 130-146 (2011-02-02)
In trees, production of intercellular signals and accessibility of signal conduits jointly govern dormancy cycling at the shoot apex. We identified 10 putative cell wall 1,3-β-glucanase genes (glucan hydrolase family 17 [GH17]) in Populus that could turn over 1,3-β-glucan (callose)
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