DR1089

Anti-NKX2-5 Mouse mAb (1E4-G5)

liquid, clone 1E4-G5, Calbiochem^®

• Synonym(s): Anti-NK2 Transcription Factor Related 5
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1E4-G5, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NKX2-5(1482)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~25-35 kDa NKX2-5 protein.

Recognizes the ~25-35 kDa NKX2-5 protein in HeLa cells and human thyroid tissue.

This Anti-NKX2-5 Mouse mAb (1E4-G5) is validated for use in ELISA, Immunoblotting for the detection of NKX2-5.

Immunogen

Full-length, recombinant, human NKX2-5 (aa 1-325), expressed as a GST fusion protein

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
Human thyroid tissue, HeLa cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Yadava, R,S., et al. 2008. Nat. Genet.40 61.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable