ABT45
Anti-Claudin-5 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, horse, human
species reactivity (predicted by homology)
equine (based on 100% sequence homology), ox (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CLDN5(7122)
General description
Claudin-5, a transmembrane protein deleted in velo-cardio-facial syndrome (TMVCF), seems to be involved in endothelial tight junctions. Claudin-5 has been exclusively found in the cell-cell borders of endothelial cells. Lower claudin-5 levels in sinusoidal endothelial cells could lead to their dysfunction during liver injuries. Levels of claudin-5 in tumor vessels could be a potential marker for hepatocellular carcinoma prognosis. The loss of claudin-5 function has been linked to carcinogenesis.
Specificity
This antibody recognizes Claudin-5 at the cytoplasmic domain.
Immunogen
Epitope: Cytoplasmic Domain
KLH-conjugated linear peptide corresponding to human Claudin-5 at the cytoplasmic domain.
Application
Anti-Claudin-5 Antibody detects level of Claudin-5 & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:800 dilution from a representative lot detected Clausin-5 in prostate tissue.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Quality
Evaluated by Western Blot in human lung tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Claudin-5 in 10 µg of human lung tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Claudin-5 in 10 µg of human lung tissue lysate.
Target description
~17 kDa observed.
Molecular weights at ~17 kDa and 62 kDa may be observed depending on phosphorylation and glycosylation effects (Ishizaki, T., et al. (2003). Experimental Cell Research. 290:275–288).
Molecular weights at ~17 kDa and 62 kDa may be observed depending on phosphorylation and glycosylation effects (Ishizaki, T., et al. (2003). Experimental Cell Research. 290:275–288).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human lung tissue lysate
Human lung tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Haitao Liu et al.
Diabetologia, 62(12), 2365-2374 (2019-10-16)
Levels of neutrophil elastase, a serine protease secreted by neutrophils, are elevated in diabetes. The purpose of this study was to determine whether neutrophil elastase (NE) contributes to the diabetes-induced increase in retinal vascular permeability in mice with streptozotocin-induced diabetes
Disrupted tight junctions in the small intestine of cystic fibrosis mice.
De Lisle, RC
Cell and Tissue Research null
Zhi Fang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30(6), 2097-2107 (2016-02-19)
The mechanism of blood-brain barrier (BBB) disruption, involved in poststroke edema and hemorrhagic transformation, is important but elusive. We investigated microRNA-150 (miR-150)-mediated mechanism in the disruption of BBB after stroke in rats. We found that up-regulation of miR-150 increased permeability
Hongyan Zhu et al.
Neurochemical research, 43(10), 1897-1904 (2018-08-09)
Blood-brain barrier (BBB) destruction is associated with a variety of neurological diseases. Brain microvascular endothelial cells (BMECs) are the key constituent of BBB. Both matrix metalloproteinases-2/9 (MMP-2/9) and toll-like receptor-2 (TLR2) are coexpressed in BMECs and have been shown to
Karsten Bartels et al.
Heart, lung & circulation, 23(10), 981-984 (2014-06-17)
Neurologic injury is common after cardiac surgery and disruption of the blood brain barrier (BBB) has been proposed as a contributing factor. We sought to study BBB characteristics in a rodent model of cardiopulmonary bypass (CPB) and deep hypothermic circulatory
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