14-746M-M

Src (1-530) Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, human Src, amino acids 1-530, expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.

• UNSPSC Code: 12352200
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• mol wt: M_w 61.7 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• GenBank accession no.: K03218
• UniProt accession no.: P05480 (mouse); P12931; Q9WUD9 (rat)
• Gene Information: human ... SRC(6714)

Description

General description

N-terminal 6His-tagged, recombinant, human Src, amino acids 1-530, expressed by baculovirus in Sf21 insect cells.

Product Source: Expressed by baculovirus in Sf21 insect cells.

The mutant form of Src (Src, 1-530) has been identified in cases of advanced colon cancer. This truncating mutation generates an open active form of Src that has been found to be transforming, tumorigenic and metastasis promoting.

Application

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Biochem/physiol Actions

Protein Target: Src

Target Sub-Family: TK

Packaging

Also available in 10 μg size (cat#14-746) and in bulk (cat# 14-746-K).

Physical form

50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution

Ni²⁺/NTA-agarose

Storage and Stability

6 months at -70°C from date of shipment.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable