跳转至内容
Merck
HomeAdvanced Gene EditingCRISPR Cas9 Proteins

CRISPR Cas9 Proteins

A Cas9 protein and synthetic guide RNA form a complex—a ribonucleoprotein (RNP)— a key to successful gene editing. Direct introduction of an RNP, rather than expression from a plasmid, offers many benefits: a functional nuclease is immediately available in the cell, then the complex is quickly cleared, making the editing activity short-lived. This reduces off-target effects and leaves no genomic footprint from DNA integration into the genome.

Choosing the best Cas9 protein for your specific application will ensure success in your experimental goals. Whether you need a Cas9 protein with very low off-target effects, one appropriate for gene activation/inhibition studies, or one allowing visualization of delivery, we offer options that will work for you. We have the largest selection of Cas9 proteins available, so we can meet these, and many other specifications.

Some features of our Cas9 proteins include:

  • More nuclear localization signals (NLSs) for specificity
  • Stringent manufacturing and purification standards
  • High editing activity and specificity – don’t compromise!
  • Reduced off- targeting with transient expression

Once you have selected your Cas9 protein, browse our RNP protocols to see exactly how these powerful tools can work for you.

Click here for additional information on Cas9-GFP proteins.

Design and order your gRNAs to complete your RNP complex:


Available Proteins

WT Cas9Cas9-GFPCas9 PlusCas9 D10A (Nickase)Cas9 D10A H840A (Dead)PURedit™ Cas9
DescriptionWild-type SpCas9 protein with high gene editing activityWild-type SpCas9 protein fused to GFP with high gene editing activitySpCas9 protein engineered to have both high gene editing activity and on-target specificityCas9 with mutation to limit cleavage to a single strand, creating a nick in DNA; requires 2 target sites for modificationCas9 with mutations to inhibit nuclease activity but allow binding to DNASpCas9 protein engineered to have both highest gene editing activity and on-target specificity
Recommended UsageGo-to for early evaluation when gRNA targets are screened for efficacyBeneficial for optimizing experimental conditions
Evaluate new delivery mechanisms
Ideal for gene editing experiments that require low off-target effects without compromising on-target activityMost helpful for homology-directed repair (HDR) applicationsGene modification experiments that do not require cutting the DNA – Inhibition or Activation of gene targetPreclinical research where high efficiency and high quality are required
Features1× NLS
Lyophilized
3× NLS
Enhanced GFP
Lyophilized
Activity & Specificity Enhancing mutations
1× NLS
Lyophilized
D10A mutation
1× NLS
Lyophilized
D10A & H840A mutations
1× NLS
3× FLAG™-Biotin Tag
Lyophilized
Activity & Specificity Enhancing mutations
1× NLS
Lyophilized
Gene Editing Activity+++++++++++N/A++++
On-Target Specificity+++++++++++N/A++++
Price++++++++++
Quality StandardISO 9001ISO 9001ISO 9001ISO 9001ISO 9001ISO 9001 with enhanced quality control required for preclinical & early clinical research
Product NumberCAS9PROTCAS9GFPPROCAS9PLCAS9D10APRDCAS9PROTPECAS9



Search to order Pre-designed and Custom gRNAs



Highlights

Gene Editing 101 eBook

A free eBook designed to help you understand, design, and perform CRISPR experiments. Download your copy now!

Video - The Core Benefits of CRISPR

Cas9-GFP Proteins - Visualize your CRISPR experiment.

登录以继续。

如要继续阅读,请登录或创建帐户。

暂无帐户?