CRISPR Cas9 Proteins
A Cas9 protein and synthetic guide RNA form a complex—a ribonucleoprotein (RNP)— a key to successful gene editing. Direct introduction of an RNP, rather than expression from a plasmid, offers many benefits: a functional nuclease is immediately available in the cell, then the complex is quickly cleared, making the editing activity short-lived. This reduces off-target effects and leaves no genomic footprint from DNA integration into the genome.
Choosing the best Cas9 protein for your specific application will ensure success in your experimental goals. Whether you need a Cas9 protein with very low off-target effects, one appropriate for gene activation/inhibition studies, or one allowing visualization of delivery, we offer options that will work for you. We have the largest selection of Cas9 proteins available, so we can meet these, and many other specifications.
Some features of our Cas9 proteins include:
- More nuclear localization signals (NLSs) for specificity
- Stringent manufacturing and purification standards
- High editing activity and specificity – don’t compromise!
- Reduced off- targeting with transient expression
Once you have selected your Cas9 protein, browse our RNP protocols to see exactly how these powerful tools can work for you.
Click here for additional information on Cas9-GFP proteins.
Design and order your gRNAs to complete your RNP complex:
Available Proteins | ||||||
---|---|---|---|---|---|---|
WT Cas9 | Cas9-GFP | Cas9 Plus | Cas9 D10A (Nickase) | Cas9 D10A H840A (Dead) | PURedit™ Cas9 | |
Description | Wild-type SpCas9 protein with high gene editing activity | Wild-type SpCas9 protein fused to GFP with high gene editing activity | SpCas9 protein engineered to have both high gene editing activity and on-target specificity | Cas9 with mutation to limit cleavage to a single strand, creating a nick in DNA; requires 2 target sites for modification | Cas9 with mutations to inhibit nuclease activity but allow binding to DNA | SpCas9 protein engineered to have both highest gene editing activity and on-target specificity |
Recommended Usage | Go-to for early evaluation when gRNA targets are screened for efficacy | Beneficial for optimizing experimental conditions Evaluate new delivery mechanisms | Ideal for gene editing experiments that require low off-target effects without compromising on-target activity | Most helpful for homology-directed repair (HDR) applications | Gene modification experiments that do not require cutting the DNA – Inhibition or Activation of gene target | Preclinical research where high efficiency and high quality are required |
Features | 1× NLS Lyophilized | 3× NLS Enhanced GFP Lyophilized | Activity & Specificity Enhancing mutations 1× NLS Lyophilized | D10A mutation 1× NLS Lyophilized | D10A & H840A mutations 1× NLS 3× FLAG™-Biotin Tag Lyophilized | Activity & Specificity Enhancing mutations 1× NLS Lyophilized |
Gene Editing Activity | +++ | +++ | +++ | ++ | N/A | ++++ |
On-Target Specificity | ++ | ++ | ++++ | +++ | N/A | ++++ |
Price | + | ++ | ++ | + | + | +++ |
Quality Standard | ISO 9001 | ISO 9001 | ISO 9001 | ISO 9001 | ISO 9001 | ISO 9001 with enhanced quality control required for preclinical & early clinical research |
Product Number | CAS9PROT | CAS9GFPPRO | CAS9PL | CAS9D10APR | DCAS9PROT | PECAS9 |
Search to order Pre-designed and Custom gRNAs
Highlights
A free eBook designed to help you understand, design, and perform CRISPR experiments. Download your copy now!
Video - The Core Benefits of CRISPR
Cas9-GFP Proteins - Visualize your CRISPR experiment.
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