Trace HPLC Analysis of Proline Enantiomers on Astec^® CLC-L

column

Astec CLC-L, 15 cm x 4.6 mm I.D., 5 μm particles (53123AST)

mobile phase

5 mM copper sulfate

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

10 μL

sample

1 mg/mL in water