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Merck

LC/MS (TOF) Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC

LC/MS (TOF) Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC application for HPLC

材料

分析柱

产品编号
说明
价格

53939-U

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

標準

产品编号
说明
价格

D-055

N-Desethylamiodarone hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

F-017

(±)-Flecainide solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)

mobile phase

[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile; (5:95, A:B, pH 7.0 with formic acid)

flow rate

0.4 mL/min

column temp.

35 °C

detector

ESI(+), full scan, m/z 200-800

injection

0.5 μL

sample

each compound, 300 ng/mL in 1% formic acid acetonitrile:water, 75:25

说明

分析報告

The basic nature of these compounds makes them targets for HILIC chromatographic separation. HILIC mobile phases consist of a high composition of acetonitrile, which facilitates the direct analysis of precipitated plasma samples without the need for additional sample solvent exchange. In most cases, the high organic mobile phase also facilitates increased analyte response with ESI(+) MS detection. Separation was performed on an Ascentis Express HILIC Fused-Core HPLC column. High purity solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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