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LC/MS Analysis of Methadone and Metabolites EDDP and EMDP on Ascentis® Express RP Amide

LC/MS Analysis of Methadone and Metabolites EDDP and EMDP on Ascentis® Express RP Amide application for HPLC

材料

分析柱

产品编号
说明
价格

53913-U

Ascentis® Express RP-Amide 2.7μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

標準

产品编号
说明
价格

M-007

(+/-)-美沙酮标准液 溶液

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

E-022

EDDP高氯酸盐标准液 CRM 溶液

1.0 mg/mL in methanol (as pyrolinium), ampule of 1 mL, certified reference material, Cerilliant®

E-057

EMDP盐酸盐标准液 盐酸盐 溶液

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express RP Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)

mobile phase

[A] 10 mM ammonium formate in water, pH 3.6; [B] 10 mM ammonium formate in acetonitrile, pH 3.6; (65:35, A:B)

flow rate

0.5 mL/min

column temp.

35 °C

detector

ESI(+), m/z 100-1000

injection

0.5 μL

sample

200 μg/L in 25:75, water: acetonitrile

说明

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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