HPLC Analysis of Selegiline and Amphetamine Metabolites on Discovery^® HS F5

column

Discovery HS F5, 5 cm × 4.6 mm I.D., 5 μm particles (567513-U)

mobile phase

[A] water (10 mM ammonium acetate, pH 4.0 with acetic acid: [B] acetonitrile (v/v), (25:75, A:B)

flow rate

1.0 mL/min

column temp.

40 °C

detector

UV, 210 nm

injection

10 μL

sample

50 μg/mL each in mobile phase