HPLC Analysis of Peroxidase on TSKgel^® G3000SWXL

column

TSKgel G3000SW_XL, 30 cm × 7.8 mm I.D., 5 μm particles (808541)

mobile phase

0.3M sodium chloride/0.05M phosphate buffer, pH 7.0

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 220 nm

injection

100 μL crude peroxidase preparation from Japanese horseradish (0.15mg)