HPLC Analysis of Ascorbic Acid on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

25 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 230 nm

injection

10 μL

sample

as indicated each in mobile phase