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HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection)

HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection) application for HPLC

材料

分析柱

产品编号
说明
价格

Ascentis® Express RP-Amide 2.7μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 5 cm × 3 mm

標準

产品编号
说明
价格

咖啡因

Sigma Reference Standard, vial of 250 mg

山梨酸

99.0-101.0% anhydrous basis

盐酸吡哆素 (B6)

analytical standard

尼克酰胺(烟酰胺)

analytical standard

核黄素 (B2)

analytical standard

SS苯甲酸

2000 μg/mL in dichloromethane, analytical standard

CONDITIONS

column

Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)

mobile phase

[A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA

flow rate

0.6 mL/min

pressure

1146 psi (79 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Energy Drink Diluted 1:9 in acetonitrile

说明

分析報告

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in caffeinated energy drinks.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany