HPLC Analysis of 4-Phenyl-2-oxazolidinone Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] 0.5% triethylamine acetate, pH 4.1; [B] methanol; (80:20, A:B)

flow rate

1 mL/min

column temp.

ambient

detector

UV, 254 nm

injection

5 μL

sample

5 mg/mL in methanol