- Automated Solid-Phase Click Synthesis of Oligonucleotide Conjugates: From Small Molecules to Diverse N-Acetylgalactosamine Clusters.
Automated Solid-Phase Click Synthesis of Oligonucleotide Conjugates: From Small Molecules to Diverse N-Acetylgalactosamine Clusters.
Bioconjugate chemistry (2017-09-19)
Valentina M Farzan, Egor A Ulashchik, Yury V Martynenko-Makaev, Maksim V Kvach, Ilya O Aparin, Vladimir A Brylev, Tatiana A Prikazchikova, Svetlana Yu Maklakova, Alexander G Majouga, Alexey V Ustinov, German A Shipulin, Vadim V Shmanai, Vladimir A Korshun, Timofei S Zatsepin
PMID28921968
摘要
We developed a novel technique for the efficient conjugation of oligonucleotides with various alkyl azides such as fluorescent dyes, biotin, cholesterol, N-acetylgalactosamine (GalNAc), etc. using copper-catalysed alkyne-azide cycloaddition on the solid phase and CuI·P(OEt)