跳转至内容
Merck
  • The bacterial toxin CNF1 as a tool to induce retinal degeneration reminiscent of retinitis pigmentosa.

The bacterial toxin CNF1 as a tool to induce retinal degeneration reminiscent of retinitis pigmentosa.

Scientific reports (2016-10-25)
Viviana Guadagni, Chiara Cerri, Ilaria Piano, Elena Novelli, Claudia Gargini, Carla Fiorentini, Matteo Caleo, Enrica Strettoi
摘要

Retinitis pigmentosa (RP) comprises a group of inherited pathologies characterized by progressive photoreceptor degeneration. In rodent models of RP, expression of defective genes and retinal degeneration usually manifest during the first weeks of postnatal life, making it difficult to distinguish consequences of primary genetic defects from abnormalities in retinal development. Moreover, mouse eyes are small and not always adequate to test pharmacological and surgical treatments. An inducible paradigm of retinal degeneration potentially extensible to large animals is therefore desirable. Starting from the serendipitous observation that intraocular injections of a Rho GTPase activator, the bacterial toxin Cytotoxic Necrotizing Factor 1 (CNF1), lead to retinal degeneration, we implemented an inducible model recapitulating most of the key features of Retinitis Pigmentosa. The model also unmasks an intrinsic vulnerability of photoreceptors to the mechanism of CNF1 action, indicating still unexplored molecular pathways potentially leading to the death of these cells in inherited forms of retinal degeneration.

材料
货号
品牌
产品描述

Sigma-Aldrich
抗胶质纤维酸性蛋白 兔抗, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
二氢乙锭, BioReagent, suitable for fluorescence, ≥95% (HPCE)
Sigma-Aldrich
单克隆抗-视蛋白 小鼠抗, clone RET-P1, ascites fluid
Sigma-Aldrich
抗-蛋白激酶 Cα 兔抗, whole antiserum
Sigma-Aldrich
单克隆抗 蛋白激酶 C (PKC) 小鼠抗, clone MC5, ascites fluid
Sigma-Aldrich
Anti-phospho-LIMK1 (pThr508) antibody produced in rabbit, affinity isolated antibody