- Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris.
Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris.
Journal of structural biology (2013-07-09)
Hermann Ehrlich, Oksana V Kaluzhnaya, Eike Brunner, Mikhail V Tsurkan, Alexander Ereskovsky, Micha Ilan, Konstantin R Tabachnick, Vasilii V Bazhenov, Silvia Paasch, Martin Kammer, René Born, Allison Stelling, Roberta Galli, Sergei Belikov, Olga V Petrova, Victor V Sivkov, Denis Vyalikh, Sebastian Hunoldt, Gert Wörheide
PMID23831449
摘要
This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques ((13)C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.
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