A colloidal gold nanoparticle-based immunochromatographic test strip for rapid and convenient detection of Staphylococcus aureus.

A colloidal gold nanoparticle-based immunochromatographic test strip for rapid and convenient detection of Staphylococcus aureus.

Journal of nanoscience and nanotechnology (2014-04-25)

Kaili Niu, Xiaoping Zheng, Chusen Huang, Kuan Xul, Yuan Zhi, Hebai Shen, Nengqin Jia

摘要

An immunochromatographic test strip using gold nanoparticles-staphylococcus aureus monoclonal antibody conjugates was developed for the rapid and convenient detection of staphylococcus aureus based on a double-antibody sandwich format. The detection limit and the detection rate of this test strip is 10(3) CFU /mL and 98.7%, respectively. It could be used for the rapid detection of staphylococcus aureus in food and the results can be visually identified by the naked eye within 10 min. Compared with conventional bacterial detection methods, this developed immunochromatographic assay based test strip has several advantages including simple, fast, low-cost, favorable sensitivity and specificity, exhibiting a great potential for application in food safety control systems and clinical diagnosis.

材料

货号

品牌

产品描述

265772

Sigma-Aldrich

金, powder, <45 μm, 99.99% trace metals basis

636347

Sigma-Aldrich

金, nanopowder, <100 nm particle size, 99.9% trace metals basis

326585

Sigma-Aldrich

金, powder, <10 μm, ≥99.9% trace metals basis

265810

Sigma-Aldrich

金, foil, thickness 0.1 mm, 99.99% trace metals basis

643203

Sigma-Aldrich

镀金显微镜载片, 99.999% (Au), layer thickness 100 Å

326534

Sigma-Aldrich

金, wire, diam. 0.25 mm, ≥99.9% trace metals basis

265799

Sigma-Aldrich

金, wire, diam. 1.0 mm, 99.99% trace metals basis

265829

Sigma-Aldrich

金, foil, thickness 0.5 mm, 99.99% trace metals basis

326542

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金, beads, 1-6 mm, 99.999% trace metals basis

349283

Sigma-Aldrich

金, wire, diam. 0.1 mm, 99.99% trace metals basis

326496

Sigma-Aldrich

金, foil, thickness 0.127 mm, 99.99% trace metals basis

643246

Sigma-Aldrich

镀金显微镜载片, 99.999% (Au), layer thickness 1000 Å

643254

Sigma-Aldrich

镀金盖玻片, 99.999% (Au), layer thickness 100 Å, L × W × thickness 22 mm × 22 mm × 130-170 μm, square

310980

Sigma-Aldrich

金, wire, diam. 0.5 mm, 99.99% trace metals basis

373184

Sigma-Aldrich

金, evaporation slug, diam. × L 0.3 cm × 0.6 cm, 99.99% trace metals basis

349275

Sigma-Aldrich

金, foil, thickness 0.05 mm, 99.99% trace metals basis

GF96742479

金, rod, 50mm, diameter 3.0mm, as drawn, 99.95%

265837

Sigma-Aldrich

金, rod, diam. 3.0 mm, 99.99% trace metals basis

268461

Sigma-Aldrich

金, foil, thickness 0.025 mm, 99.99% trace metals basis

349240

Sigma-Aldrich

金, foil, thickness 0.25 mm, ≥99.9% trace metals basis

GF77657574

金, sphere, 20pcs, diameter 0.5mm, 99.99%

349305

Sigma-Aldrich

金, wire, diam. 1.0 mm, 99.997% trace metals basis

265780

Sigma-Aldrich

金, wire, diam. 0.25 mm, 99.99% trace metals basis

349313

Sigma-Aldrich

金, wire, diam. 0.5 mm, 99.999% trace metals basis

255734

Sigma-Aldrich

金, powder, <850 μm, ≥99.99% trace metals basis

373168

Sigma-Aldrich

金, evaporation slug, diam. × L 0.6 cm × 0.6 cm, 99.99% trace metals basis

643289

Sigma-Aldrich

镀金盖玻片, 99.999% (Au), diam. × thickness 15 mm × 130-170 μm, layer thickness 100 Å

GF41388767

金, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%

GF06766713

金, insulated wire, 5m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%

GF62399133

金, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%