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A colloidal gold nanoparticle-based immunochromatographic test strip for rapid and convenient detection of Staphylococcus aureus.

A colloidal gold nanoparticle-based immunochromatographic test strip for rapid and convenient detection of Staphylococcus aureus.

Journal of nanoscience and nanotechnology (2014-04-25)

Kaili Niu, Xiaoping Zheng, Chusen Huang, Kuan Xul, Yuan Zhi, Hebai Shen, Nengqin Jia

摘要

An immunochromatographic test strip using gold nanoparticles-staphylococcus aureus monoclonal antibody conjugates was developed for the rapid and convenient detection of staphylococcus aureus based on a double-antibody sandwich format. The detection limit and the detection rate of this test strip is 10(3) CFU /mL and 98.7%, respectively. It could be used for the rapid detection of staphylococcus aureus in food and the results can be visually identified by the naked eye within 10 min. Compared with conventional bacterial detection methods, this developed immunochromatographic assay based test strip has several advantages including simple, fast, low-cost, favorable sensitivity and specificity, exhibiting a great potential for application in food safety control systems and clinical diagnosis.

材料

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品牌

产品描述

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金, powder, <45 μm, 99.99% trace metals basis

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金, powder, <10 μm, ≥99.9% trace metals basis

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金, nanopowder, <100 nm particle size, 99.9% trace metals basis

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金, wire, diam. 0.25 mm, ≥99.9% trace metals basis

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金, evaporation slug, diam. × L 0.6 cm × 0.6 cm, 99.99% trace metals basis

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金, foil, thickness 0.025 mm, 99.99% trace metals basis

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金, foil, thickness 0.127 mm, 99.99% trace metals basis

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金, beads, 1-6 mm, 99.999% trace metals basis

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镀金显微镜载片, layer thickness 100 Å, 99.999% (Au)

金, rod, 50mm, diameter 3.0mm, as drawn, 99.95%

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金, wire, diam. 0.5 mm, 99.99% trace metals basis

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金, wire, diam. 1.0 mm, 99.997% trace metals basis

金, rod, 10mm, diameter 3.0mm, as drawn, 99.95%

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镀金显微镜载片, layer thickness 1000 Å, 99.999% (Au)

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金, foil, thickness 0.5 mm, 99.99% trace metals basis

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金, rod, diam. 3.0 mm, 99.99% trace metals basis

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金, powder, <850 μm, ≥99.99% trace metals basis

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金, evaporation slug, diam. × L 0.3 cm × 0.6 cm, 99.99% trace metals basis

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镀金盖玻片, 99.999% (Au), L × W × thickness 22 mm × 22 mm × 130-170 μm, square, layer thickness 100 Å

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金, rod, 6mm, diameter 6.0mm, as drawn, 99.95%

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金, foil, thickness 0.05 mm, 99.99% trace metals basis

金, sphere, 20pcs, diameter 0.5mm, 99.99%

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