- Use of cross-linking in studying the structure of RNA tumour viruses.
Use of cross-linking in studying the structure of RNA tumour viruses.
Acta virologica (1976-08-01)
G Russ, K Poláková, V Thurzo
PMID9823
摘要
Treatment of intact avian myeloblastosis virus (AMV) with dimethyl suberimidate dihydrochloride (DMS), a cross-linking agent specific for amino groups, was found to result in progressive cross-linking among viral proteins, as revealed by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulphate (SDS). Free viral proteins were not cross-linked. The cross-linked protein complex with an apparent molecular weight of 50,000 daltons was studied in detail.
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