- Enzyme electrode for the measurement of lactate in undiluted blood.
Enzyme electrode for the measurement of lactate in undiluted blood.
Clinica chimica acta; international journal of clinical chemistry (1986-06-15)
W H Mullen, S J Churchouse, F H Keedy, P M Vadgama
PMID3719998
摘要
An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H2O2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l L-lactate with response times of 1-3 min, whilst giving independence from stirring. The method is suitable for the bedside analysis of a single drop of undiluted whole blood. Correlation with a routine method gave y = 0.96x + 0.11, r = 0.996, n = 46.