- Toxoflavin lyase enzyme as a marker for selecting potato plant transformants.
Toxoflavin lyase enzyme as a marker for selecting potato plant transformants.
Bioscience, biotechnology, and biochemistry (2012-12-12)
Mi-Sun Kim, HyunSoon Kim, Jae Sun Moon, Ingyu Hwang, Hyouk Joung, Jae-Heung Jeon
PMID23221711
摘要
This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.