Impact of light quality and space weather in Alexandrium catenella (Dinophyceae) cultures.

The chain-forming dinoflagellate Alexandrium catenella was grown with LED or fluorescent light. With LED light, the pool of mycosporine-like amino acids (MAAs) with strong antioxidant properties, such as mycosporine-glycine and palythene, was reduced in comparison to fluorescent light. The conjugated MAAs M-320 and M-335/360 presented similar stability to light and oxidation than their respective more sensitive moiety: mycosporine-glycine and palythene, respectively. A. catenella was exposed to extracellular hydrogen peroxide under LED or fluorescent light. It triggered an increase in chain formation at a final concentration of 98 and 196 µM, typical of an inverted U-shaped hormetic response, and reduced cell survival above 294 µM. LED light, minimizing UV-stress photoprotection, was chosen to render cells more susceptible to space weather. Cultures were exposed to 490 µM H2O2 multiple times between November 2017 and February 2018, close to the minimum of solar cycle 24. Cell survival was dependent on temperature, geomagnetic activity, solar X-rays and neutron flux. Geomagnetic activity originated an inverted U-shaped survival curve and X-ray flux a J-shaped survival curve, this second type of hormetic response being more rarely found. The percentage of cells in chains increased moderately with temperature, and more significantly with solar X-ray flux. Chain formation reduced along culture growth, but these observations were dependent on the occurring X-ray flux.