1X TBS Working Solution for Western Blotting
Tris-buffered saline (TBS) is an excellent wash buffer for many types of immunoassays, such as ELISAs, immunohistochemisty, and Western blots. To make 1 L of TBS wash buffer, add 100 mL of 10X TBS to 900 mL of water.
This calculator enables the accurate preparation of a 1X TBS working solution whether you are making enough for a single experiment or for the entire lab. Input your desired volume, click the CALCULATE button, and the table will populate with the amount of each component needed.
1X Solution concentrations:
- Tris: 20 mM
- NaCl: 150 mM
TBS Recipe Calculator
OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS AND SOLUTIONS
Stock Solutions
- 4X SDS Sample Loading Buffer
- 10X Phosphate-Buffered Saline (PBS)
- 10X Tris-Buffered Saline (TBS) Stock Solution
- Stock Components for Western Blot Stripping Solutions
Wash & Blocking Buffers
- 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
- 1X Phosphate-Buffered Saline (PBS)
- 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
- Non-Fat Dry Milk (NFDM) Blocking Buffer
- Bovine Serum Albumin (BSA) Blocking Buffer
Stripping Solutions
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